Ian, N. Schwartz, M. A. Integrin-mediated adhesion regulates membrane order. J Cell Biol 174, 725?34 (2006). 41. Cary, L. A., Klinghoffer, R. A., Sachsenmaier, C. Cooper, J. A. SRC catalytic but not scaffolding function is required for integrin-regulated tyrosine phosphorylation, cell migration, and cell spreading. Mol Cell Biol 22, 2427?440 (2002). 42. Mitra, S. K. Schlaepfer, D. D. Integrin-regulated FAK-Src signaling in normal and cancer cells. Curr Opin Cell Biol 18, 516?23 (2006). 43. Thomas, S. M. Brugge, J. S. Cellular functions regulated by Src loved ones kinases. Annu Rev Cell Dev Biol 13, 513?09 (1997). 44. Berdeaux, R. L., Diaz, B., Kim, L. Martin, G. S. Active Rho is localized to podosomes induced by oncogenic Src and is required for their assembly and function. J Cell Biol 166, 317?23 (2004).Price of 3-Bromo-5-hydroxybenzonitrile 45. Parsons, J. T., Horwitz, A. R. Schwartz, M. A. Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nat Rev Mol Cell Biol 11, 633?43, doi:10.1038/nrm2957 (2010). 46. Quizi, J. L. et al. SLK-mediated phosphorylation of paxillin is essential for focal adhesion turnover and cell migration. Oncogene doi:10.1038/onc.2012.488 (2012). 47. Margadant, C., Monsuur, H. N., Norman, J. C. Sonnenberg, A. Mechanisms of integrin activation and trafficking. Curr Opin Cell Biol 23, 607?14, doi:10.1016/ j.ceb.2011.08.005 (2011). 48. Schneller, M., Vuori, K. Ruoslahti, E. Alphavbeta3 integrin associates with activated insulin and PDGFbeta receptors and potentiates the biological activity of PDGF. Embo J 16, 5600?607, doi:ten.1093/emboj/16.18.5600 (1997). 49. Arias-Salgado, E. G. et al. Src kinase activation by direct interaction together with the integrin beta cytoplasmic domain. Proc Natl Acad Sci U S A 100, 13298?3302, doi:ten.1073/pnas.2336149100 (2003). 50. Boggon, T. J. Eck, M. J. Structure and regulation of Src loved ones kinases. Oncogene 23, 7918?927 (2004). 51. Woodard, A. S. et al. The synergistic activity of alphavbeta3 integrin and PDGF receptor increases cell migration. J Cell Sci 111 (Pt four), 469?78 (1998). 52. Rossier, O. et al. Integrins beta1 and beta3 exhibit distinct dynamic nanoscale organizations inside focal adhesions. Nat Cell Biol 14, 1057?067, doi:10.1038/ ncb2588 (2012). 53. Lu, S. et al. Computational analysis with the spatiotemporal coordination of polarized PI3K and Rac1 activities in micro-patterned live cells. PLoS One six, e21293, doi:ten.1371/journal.pone.0021293 (2011). 54. Balaban, N. Q. et al. Force and focal adhesion assembly: a close connection studied using elastic micropatterned substrates. Nat Cell Biol three, 466?72 (2001). 55. Cipra, T. et al. Detection and interpolation of outliers in biosignals.334951-61-0 site Act Nerv Super (Praha) 32, 283?91 (1990).PMID:33662619 SCIENTIFIC REPORTS | four : 5756 | DOI: 10.1038/srepnature/scientificreports56. Efron, B. Tibshirani, R. J. An Introduction towards the Bootstrap. (Chapman Hall, 1993). 57. Manly, B. F. J. Randomization, Bootstrap and Monte Carlo Techniques in Biology. 2nd edn, (Chapman Hall, 1997).Additional informationSupplementary facts accompanies this paper at http://nature/ scientificreports Competing economic interests: The authors declare no competing monetary interests. How you can cite this short article: Lu, S.Y. et al. Decipher the dynamic coordination in between enzymatic activity and structural modulation at focal adhesions in living cells. Sci. Rep. 4, 5756; DOI:10.1038/srep05756 (2014). This perform is licensed under a Inventive Commons Attribution-NonCommercialShareAlike 4.0 Internati.