Product Name :
Phospho-Vimentin (S39) Recombinant Rabbit Monoclonal Antibody [JE43-26]
Predicted band size :
54 kDa
Observed band size :
54 kDa
Synonyms:
CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody
Function :
Vimentin is a structural protein that in humans is encoded by the VIM gene. Its name comes from the Latin vimentum which refers to an array of flexible rods. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phosphopeptide corresponding to residues surrounding Ser39 of human vimentin protein.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell
Coupling :
unconjugated
Clone number:
JE43-26
RRID:
AB_3072558
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Buy939793-16-5 Aliquot store at -20℃.Methyl 4-chloro-3-oxobutanoate Formula Avoid repeated freeze / thaw cycles.PMID:33576270
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 54 kDa
Positive control :
HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes.