Cells have been harvested, resuspended in TBSC (TBS pH 7.4, two mM CaCl2) with
Chemexpress Cells were harvested, resuspended in TBSC (TBS pH 7.four, 2 mM CaCl2) with protease inhibitor mix (Comprehensive, Roche Applied Science) and lysed by sonication. Just after centrifugation, the supernatant was…